Leaf Disc Lab-Part 1: DesignName: Delaney DzuckTitle: The Effects of Different Amounts of Baking Soda on the Process of Facilitating Photosynthesis.
Aim: To study how the amounts of carbon dioxide impact the photosynthesis.Background:Photosynthesis is a process that is widely known, but not well understood. “In plants, photosynthesis is used to convert light energy from sunlight into chemical energy (glucose). Carbon dioxide, water, and light are used to make glucose and oxygen”(Helmenstine, 2016) Without photosynthesis less oxygen would be created which could become a problem eventually. For this reason, it is important to know what variables could negatively impact the creation of our oxygen. Carbon dioxide is used in the process of photosynthesis. “The primary effects of high CO2 are an increase in photosynthetic rate and a decrease in transpiration rate” (Basil Acock, 1990). If a surplus of carbon dioxide is put into the process of photosynthesis it will speed up, not necessarily making more sugar (glucose).
“As it is normally present in the atmosphere at very low concentrations (about 0.04%), increasing carbon dioxide concentration causes a rapid rise in the rate of photosynthesis, which eventually plateaus when the maximum rate of fixation is reached” (RSC, 2009) At a certain point of adding carbon dioxide, the process will plateau in time and it will have reached its maximum. Hypothesis: If more baking soda is added to the water during the leaf disc lab then the photosynthesis process will go faster because the primary effects of high carbon dioxide are an increase in photosynthetic rate. Variables: Independent: the amount of baking soda (carbon dioxide)Dependent: the time in which it takes the leaf discs to reach the top of the watercontrolled variables:The size of the leaf discsThe amount of waterThe type of lightMaterials: Sodium Bicarbonate (baking soda)Liquid Soap Syringe LeafHole Punch TimerLight Source (LED bulbs)Stir sticksPlastic cups?, ¼, ½ tsp Procedure: Cut out ten leaf discs with a hole puncherPull of the back of the syringe and put all ten leaf discs inside.Pull the infiltration solution into the syringe about two or three centimeters Push the air out of the syringe and put your finger over the opening at the end and pull back so the leaves sink to the bottom of the solution.Shake the solution if needed. Pull the back off of the syringe and put the leaf discs into a cup with a solution of your choice (in this case it will be less co2, control, and more co2)*note: only do one kind at a timePut the cup under the LED light and start timingStir if needed so that the leaf discs won’t stick to each other or the sides of the cup.
Follow the chart below,Repeat two more times with the other two types of water solutionsData Table (empty):Number of Leaf Discs that Rise to the Top of the CupMinutesCup 1Cup 2Cup 31234567891011121314Works Cited:Acock, Basil. “Effects of Carbon Dioxide on Photosynthesis, Plant Growth, and Other Processes .” Science Societies, American Society of Agronomy, 2017, dl.sciencesocieties.org/publications/books/abstracts/asaspecialpubli/impactofcarbond/45?access=0=pdf.RSC. “Rate of Photosynthesis: Limiting Factors.” Rate of Photosynthesis: Limiting Factors | STEM, STEM, 2009, www.
Helmenstine, Anne Marie, Ph.D. “Photosynthesis Basics – Study Guide.” ThoughtCo, Jul.
1, 2016, thoughtco.com/photosynthesis-basics-study-guide-608181.