Bio Lab report Sample calculation for the0.5% hydrogen peroxide concentration with copper sulfate: ((37/2.75)/38) = 0.
354 conclusion: CuSO4 is a non-competitive inhibitorbecause the uncompetitive inhibitor locks the substrate in the enzyme whichprevents the enzyme from catalyzing the substrate and that would cause a decreasein the amount of free enzyme which is why the Vmax decreases as shown by thegraph. The Km is unchanged because the substrate can still bind to the activesite of the enzyme and also because the fixed amount of inactive enzyme innon-competitive inhibition does not affect the Km. Evaluation: Weakness Effects on results and how important Improvements described Shaking the flash While shaking the flash the rate of mixing is not constant and so some trials might have been mixed more than others and this could alter the results as the rate of mixing also affects the rate of reaction. Not shaking or using an electric stirrer Temperature Due to the fact that different groups performed the experiment at different locations in the classroom, for example our group performed the experiment near a window and this could have resulted in a slightly different temperature which could effect the results, as temperature effects the rate of reaction Performing the experiment in a controlled environment where the temperature is the same everywhere such as one side of the classroom.
Non-distilled water residue Between trials we used non-distilled water to wash the flask and this could have altered the concentration of the solution and added some minerals to the hydrogen peroxide. Using clean flasks after every trial.